Journal: bioRxiv

Article Title: CD47 as a potential biomarker for the early diagnosis of severe COVID-19

doi: 10.1101/2021.03.01.433404

Figure Lengend Snippet: SARS-CoV-2 infection is associated with increased CD47 levels. A) TF protein abundance in uninfected (control) and SARS-CoV-2-infected (virus) Caco-2 cells (data derived from . P-values were determined by two-sided Student’s t-test. B) CD47 and SARS-CoV-2 N protein levels and virus titres (genomic RNA determined by PCR) in SARS-CoV-2 strain FFM7 (MOI 1)-infected air-liquid interface cultures of primary human bronchial epithelial (HBE) cells and SARS-CoV-2 strain FFM7 (MOI 0.1)-infected Calu-3 cells. Uncropped blots are provided in Suppl. Figure 1. C) CD47 mRNA levels in post mortem samples from COVID-19 patients (data derived from ). P-values were determined by two-sided Student’s t-test.

Article Snippet: Detection occurred by using specific antibodies against CD47 (1:100 dilution, CD47 Antibody, anti-human, Biotin, REAfinityTM, # 130-101-343, Miltenyi Biotec), SARS-CoV-2 N (1:1000 dilution, SARS-CoV-2 Nucleocapsid Antibody, Rabbit MAb, #40143-R019, Sino Biological), and GAPDH (1:1000 dilution, Anti-G3PDH Human Polyclonal Antibody, #2275-PC-100, Trevigen).

Techniques: Infection, Quantitative Proteomics, Control, Virus, Derivative Assay

Journal: bioRxiv

Article Title: CD47 as a potential biomarker for the early diagnosis of severe COVID-19

doi: 10.1101/2021.03.01.433404

Figure Lengend Snippet: Results of the PubMed ( https://pubmed.ncbi.nlm.nih.gov ) literature search for “CD47 aging” (A) and “CD47 hypertension” (B). C) Overview figure of the data derived from the literature searches. Age-related increased CD47 levels may contribute to pathogenic conditions associated with severe COVID-19.

Article Snippet: Detection occurred by using specific antibodies against CD47 (1:100 dilution, CD47 Antibody, anti-human, Biotin, REAfinityTM, # 130-101-343, Miltenyi Biotec), SARS-CoV-2 N (1:1000 dilution, SARS-CoV-2 Nucleocapsid Antibody, Rabbit MAb, #40143-R019, Sino Biological), and GAPDH (1:1000 dilution, Anti-G3PDH Human Polyclonal Antibody, #2275-PC-100, Trevigen).

Techniques: Derivative Assay

Journal: bioRxiv

Article Title: CD47 as a potential biomarker for the early diagnosis of severe COVID-19

doi: 10.1101/2021.03.01.433404

Figure Lengend Snippet: Results of the PubMed ( https://pubmed.ncbi.nlm.nih.gov ) literature search for “CD47 diabetes” (A). B) Overview figure of the data derived from the literature search. Hyperglycaemia- and diabetes-induced increased CD47 levels may contribute to immune escape of SARS-CoV-2-infected cells.

Article Snippet: Detection occurred by using specific antibodies against CD47 (1:100 dilution, CD47 Antibody, anti-human, Biotin, REAfinityTM, # 130-101-343, Miltenyi Biotec), SARS-CoV-2 N (1:1000 dilution, SARS-CoV-2 Nucleocapsid Antibody, Rabbit MAb, #40143-R019, Sino Biological), and GAPDH (1:1000 dilution, Anti-G3PDH Human Polyclonal Antibody, #2275-PC-100, Trevigen).

Techniques: Derivative Assay, Infection

Journal: Blood

Article Title: Galectin-5 is bound onto the surface of rat reticulocyte exosomes and modulates vesicle uptake by macrophages.

doi: 10.1182/blood-2009-07-231449

Figure Lengend Snippet: Figure 1. Galectin-5 is present on the surface of rat red cells. (A) Freshly isolated reticulocytes or erythrocytes were adsorbed on glass coverslips and processed for immunofluorescence as described in “Fluorescence-activated cell-sorting analysis of exosomes and red cells, fluorescence microscopy of red cells.” Transmission images of red cells (left) and corresponding fluorescence imaging (right) were recorded on cells by the use of purified rabbit anti–galectin-5 antibody followed by incubation withAlexa 488 anti–rabbit antibody. (B) Young reticulocytes and erythrocytes were analyzed by flow cytometry by the use of antibodies raised against Gal-2 (dashed line), Gal-4 (dotted line), and Gal-5 (solid line), already tested for their specificity (top), or the produced anti–galectin-5 serum (solid line) and the preimmune serum (bottom, dashed line). Tinted patterns indicate cell labeling obtained in the absence of primary antibodies. (C) Lymphocytes isolated from rat blood, as described in “Cells,” were analyzed by flow cytometry for Gal-5 (left, solid line), CD47 (middle, solid line) and Syto 16 green (right, solid line). Tinted patterns indicate cell labeling in the absence of primary antibodies. (D) Ghost and raft extracts isolated from reticulocytes or mature erythrocytes, as described in “Red cell subcellular fractionation,” were processed by SDS-PAGE and analyzed by Western blot for the indicated proteins. The molecular mass (kDa) standards are indicated on the left.

Article Snippet: Mouse anti–rat CD47 was from Serotec Limited.

Techniques: Isolation, Fluorescence, FACS, Microscopy, Transmission Assay, Imaging, Incubation, Cytometry, Produced, Labeling, Fractionation, SDS Page, Western Blot

Journal: Nature Communications

Article Title: Airway epithelial CD47 plays a critical role in inducing influenza virus-mediated bacterial super-infection

doi: 10.1038/s41467-024-47963-5

Figure Lengend Snippet: HBECs were infected with ( + IAV ) or without ( Mock ) influenza A virus. a Immunoblot analysis of junction protein ZO-1 and surface protein CD47 at 1 day post-infection (dpi). Normalized CD47 and ZO-1 protein levels are presented as bar graphs (Mock, n = 4; + IAV, n = 4). b Representative whole-mount images of ZO-1 (white) and CD47 (red) at 1 dpi. The area where ZO-1 disconnection occurred are presented as bar graphs (Mock, n = 6; + IAV, n = 6). c Quantitative PCR (qPCR) analysis of ZO-1 and CD47 mRNAs at 1 and 3 dpi [Mock, n = 3; + IAV (1 dpi), n = 3; + IAV (3 dpi), n = 3]. d HBECs were treated with or without 10 μM NF-κB inhibitor caffeic acid phenethyl ester (CAPE) for 1 h before influenza virus infection. Immunoblot analysis of ICAM-1 and CD47 at 1 dpi. Normalized ICAM-1 and CD47 protein levels are presented as bar graphs (DMSO + Mock, n = 4; DMSO + IAV, n = 4; CAPE + Mock, n = 4; CAPE + IAV, n = 4). e Whole-mount images of influenza virus-infected HBECs. Co-staining of CD47 (red) and ciliated cell-specific marker protein Ac-α-tubulin (green, n = 4) or goblet cell-specific marker protein MUC5AC (cyan, n = 3). Percentages of CD47-positive cells are presented as bar graphs. Data are presented as mean values ± SEM. Significance was determined by unpaired two-tailed Student’s t test or one-way ANOVA with Tukey’s multiple comparisons test. n.s. not significant. Source data are provided as a Source Data file.

Article Snippet: On days 5 and 7 after viral infection, mice were injected intranasally with either IgG2a control antibodies (clone 2A3; Cat.BE0089; BioXCell) or anti-mouse CD47 antibodies (clone MIAP301; Cat.BE0270; BioXCell).

Techniques: Infection, Virus, Western Blot, Real-time Polymerase Chain Reaction, Staining, Marker, Two Tailed Test

Journal: Nature Communications

Article Title: Airway epithelial CD47 plays a critical role in inducing influenza virus-mediated bacterial super-infection

doi: 10.1038/s41467-024-47963-5

Figure Lengend Snippet: For CD47 knock-down, HBECs were transfected with scrambled shRNA ( sc-shRNA ) or shRNA targeting CD47 ( CD47 shRNA ) using a lentiviral delivery system. For CD47 neutralization, HBECs were treated with either IgG1 (MOPC-21) or α-hCD47 (B6H12.2) antibodies. a Gene expression of CD47 was analyzed using qRT-PCR (normalized by GAPDH mRNA) (sc-shRNA, n = 4; CD47 shRNA, n = 4). b Protein expression of CD47 was analyzed using immunoblotting (sc-shRNA, n = 5; CD47 shRNA, n = 5). c Paracellular FITC-dextran permeability was measured in Mock ( n = 16), Virus only (IAV, n = 16), Bacteria only ( S. aureus , n = 16), sc-shRNA + Super-infection ( n = 12), CD47 shRNA + Super-infection ( n = 6), IgG1 + Super-infection ( n = 6), and α-hCD47 antibodies (Ab) + Super-infection ( n = 8). d Trans-epithelial resistance was measured in Mock ( n = 15), IAV ( n = 14), S. aureus ( n = 13), sc-shRNA + Super-infection ( n = 13), CD47 shRNA + Super-infection ( n = 6), IgG1 + Super-infection ( n = 6), and α-hCD47 Ab + Super-infection ( n = 8). e Microscopic images of HBECs at 5 dpi. The percentage of the damaged area is presented as bar graphs (Mock, n = 3; Virus only, n = 3; Bacteria only, n = 3; Super-infection, n = 4; IgG1 + Super-infection, n = 4; α-hCD47 Ab + Super-infection, n = 4). Data are presented as mean values ± SEM. Significance was determined by unpaired two-tailed Student’s t test or one-way ANOVA with Tukey’s multiple comparisons test. N.D. not determined. Source data are provided as a Source Data file.

Article Snippet: On days 5 and 7 after viral infection, mice were injected intranasally with either IgG2a control antibodies (clone 2A3; Cat.BE0089; BioXCell) or anti-mouse CD47 antibodies (clone MIAP301; Cat.BE0270; BioXCell).

Techniques: Transfection, shRNA, Neutralization, Expressing, Quantitative RT-PCR, Western Blot, Permeability, Virus, Bacteria, Infection, Two Tailed Test

Journal: Nature Communications

Article Title: Airway epithelial CD47 plays a critical role in inducing influenza virus-mediated bacterial super-infection

doi: 10.1038/s41467-024-47963-5

Figure Lengend Snippet: a , b Influenza virus-infected HBECs ( IAV ) infected with live S. aureus or S. aureus -derived samples ( S , supernatant of S. aureus -cultured media; U , UV-killed S. aureus ; H , heat-killed S. aureus ). Paracellular FITC-dextran permeability ( a ) and trans-epithelial electrical resistance ( b ) of 7 groups: i ) Mock ( n = 3), ii ) Virus only ( n = 3), iii ) Bacteria only ( n = 3), iv ) Super-infection ( n = 3), v ) Virus with S ( n = 3), vi ) Virus with U ( n = 3), and vii ) Virus with H ( n = 3). c Whole mount image of CD47 (red) and S. aureus (green) in the influenza virus-infected HBECs at 1 dpi. An open arrow head indicates CD47 + cell without S. aureus and closed arrow heads indicate CD47 + cells with S. aureus . Co-localization of CD47 + cells and S. aureus are presented as violin plots (Mock, n = 6; + IAV, n = 6). d – g Bacterial adhesion assay. Colonization of S. aureus was assessed in HBECs inoculated with the virus (MOI 1) for 2 h before treatment with IgG1 control antibodies or α-hCD47 neutralizing antibodies (2 h), followed by S. aureus (MOI 3) infection for 3 h (IgG1 + S. aureus , n = 4; α-hCD47 Ab + S. aureus , n = 4; IgG1 + Super-infection, n = 4; α-hCD47 Ab+ Super-infection, n = 4) ( d ). Adherence of S. aureus WT (FnBP A+/B+, n = 4) and three mutant strains (FnBP A+/B–, n = 4; FnBP A–/B+, n = 4; FnBP A–/B–, n = 4) was assessed in HBECs inoculated with the virus (MOI 1) for 2 h, followed by S. aureus (MOI 3) infection for 3 h ( e ). Colonization of S. aureus WT (FnBP A+/B+) ( f ) and double deletion mutant (FnBP A–/B–) ( g ) was assessed in HBECs inoculated with the virus (MOI 1) for 2 h before treatment with IgG1 control antibodies ( n = 4) or α-hCD47 neutralizing antibodies (1, 5, and 10 μg/mL for FnBP A+/B+, and 10 μg/mL for FnBP A–/B–, n = 4 each) for 2 h, followed by S. aureus (MOI 3) infection for 3 h. h Pull-down assay using His-tagged hCD47 recombinant protein. Bacterial plating [FnBP A+/B+ and FnBP A–/B– ( n = 3 each in the absence or in the presence of His-tagged hCD47)] and immunoblot analysis were performed using supernatants and pellets after separation with α-His-Dynabeads™/DynaMag™−2 system. The graphs present the percentage of colony numbers grown in the culture of the supernatants or the pellets. Data are presented as mean values ± SEM. Significance was determined by one-way ANOVA with Tukey’s multiple comparisons test or unpaired two-tailed Student’s t test. n.s. not significant. Source data are provided as a Source Data file.

Article Snippet: On days 5 and 7 after viral infection, mice were injected intranasally with either IgG2a control antibodies (clone 2A3; Cat.BE0089; BioXCell) or anti-mouse CD47 antibodies (clone MIAP301; Cat.BE0270; BioXCell).

Techniques: Virus, Infection, Derivative Assay, Cell Culture, Permeability, Bacteria, Cell Adhesion Assay, Mutagenesis, Pull Down Assay, Recombinant, Western Blot, Two Tailed Test

Journal: Nature Communications

Article Title: Airway epithelial CD47 plays a critical role in inducing influenza virus-mediated bacterial super-infection

doi: 10.1038/s41467-024-47963-5

Figure Lengend Snippet: 6–8-weeks-old (18–21 g of body weight) FoxJ1 -Cre;floxed ( CD47 Foxj1 ), LysM -Cre;floxed ( CD47 LysM ), and control floxed ( CD47 f/f ) mice were infected with 100 PFU of influenza virus on day 0, and 1 ×10 8 CFU of S. aureus on day 7. a , b , k , l Body weight loss ( a , k ) and survival rates ( b , l ) were monitored for 29 days. The dotted line indicates the body weight exclusion cut-off. A mantel cox survival analysis was used to compare the survival rates between groups. The numbers in parenthesis represent the count of surviving mice. c , m Representative hematoxylin and eosin (H&E) staining of lung sections. The dotted lines indicate lymphocytic infiltration and arrows indicate alveolar hemorrhage. Lung injury scores are presented as violin plots in CD47 Foxj1 mice ( CD47 f/f , n = 8; CD47 Foxj1 n = 5) ( c ) and CD47 LysM mice ( CD47 f/f , n = 8; CD47 LysM , n = 8) ( m ). d – j , n – t Tissue injury parameters were measured at 24 h after bacterial infection; total cell number in BAL fluids (BALF) of CD47 Foxj1 mice ( CD47 f/f , n = 5; CD47 Foxj1 , n = 5) ( d ) and CD47 LysM mice ( CD47 f/f , n = 8; CD47 LysM , n = 10) ( n ); bacterial adherence in the lung of CD47 Foxj1 mice ( CD47 f/f , n = 10; CD47 Foxj1 , n = 11) ( e ) and CD47 LysM mice ( CD47 f/f , n = 13; CD47 LysM , n = 15) ( o ); bacterial invasion in the lung of CD47 Foxj1 mice ( CD47 f/f , n = 7; CD47 Foxj1 , n = 7) ( f ) and CD47 LysM mice ( CD47 f/f , n = 5; CD47 LysM , n = 5) ( p ); and bacterial burden in the spleen of CD47 Foxj1 mice ( CD47 f/f , n = 5; CD47 Foxj1 , n = 6) ( g ) and CD47 LysM mice ( CD47 f/f , n = 5; CD47 LysM , n = 5) ( q ); total protein concentrations in BALF of CD47 Foxj1 mice ( CD47 f/f , n = 5; CD47 Foxj1 , n = 5) ( h ) and CD47 LysM mice ( CD47 f/f , n = 8; CD47 LysM , n = 10) ( r ); and inflammatory cytokines TNF-α in BALF of CD47 Foxj1 mice ( CD47 f/f , n = 5; CD47 Foxj1 , n = 5) ( i ) and CD47 LysM mice ( CD47 f/f , n = 8; CD47 LysM , n = 10) ( s ), and IL-6 in BALF of CD47 Foxj1 mice ( CD47 f/f , n = 5; CD47 Foxj1 , n = 5) ( j ) and CD47 LysM mice ( CD47 f/f , n = 8; CD47 LysM , n = 10) ( t ). Data are presented as mean values ± SEM. Significance was determined by unpaired two-tailed Student’s t test. Source data are provided as a Source Data file.

Article Snippet: On days 5 and 7 after viral infection, mice were injected intranasally with either IgG2a control antibodies (clone 2A3; Cat.BE0089; BioXCell) or anti-mouse CD47 antibodies (clone MIAP301; Cat.BE0270; BioXCell).

Techniques: Infection, Virus, Staining, Two Tailed Test

Journal: Nature Communications

Article Title: Airway epithelial CD47 plays a critical role in inducing influenza virus-mediated bacterial super-infection

doi: 10.1038/s41467-024-47963-5

Figure Lengend Snippet: For neutralization test, 6–8-weeks-old (18–21 g of body weight) C57BL/6 WT mice were infected with 10 PFU of influenza virus on day 0 and 5 × 10 5 CFU of S. aureus on day 7. Before bacterial infection, mice were intranasally treated twice at day 5 and 7 with IgG2a control antibodies (2A3, n = 17) or α-mCD47 neutralizing antibodies (MIAP301, n = 16). a , b Body weight loss ( a ) and survival rates ( b ) were monitored for 29 days. The dotted line indicates the body weight exclusion cut-off. A mantel cox survival analysis was used to compare the survival rates between groups. The numbers in parenthesis are numbers of survived mice. c Representative hematoxylin and eosin (H&E) staining of lung sections (IgG2a + Mock, n = 4; α-mCD47 Ab + Mock, n = 4; IgG2a + Virus only, n = 7; α-mCD47 Ab + Virus only, n = 7; IgG2a + Bacteria only, n = 4; α-mCD47 Ab + Bacteria only, n = 4; IgG2a + Super-infection, n = 9; α-mCD47 Ab + Super-infection, n = 11). The dotted lines indicate lymphocytic infiltration and arrows indicate alveolar hemorrhage. Lung injury scores are presented as violin plots. d – j Tissue injury parameters were measured at 24 h after bacterial infection (IgG2a + Mock, n = 4; α-mCD47 Ab + Mock, n = 4; IgG2a + Virus only, n = 7; α-mCD47 Ab + Virus only, n = 7; IgG2a + Bacteria only, n = 4; α-mCD47 Ab + Bacteria only, n = 4; IgG2a + Super-infection, n = 7-9; α-mCD47 Ab + Super-infection, n = 7-11); total cell number in BAL fluids (BALF) ( d ), bacterial adherence ( e ) and invasion ( f ) in the lung, and the bacterial burden in the spleen ( g ), total protein concentrations ( h ) in BALF, and inflammatory cytokines TNF-α ( i ) and IL-6 ( j ) in BALF. Data are presented as mean values ± SEM. Significance was determined by unpaired two-tailed t test. n.s. not significant. N.D. not determined. Source data are provided as a Source Data file.

Article Snippet: On days 5 and 7 after viral infection, mice were injected intranasally with either IgG2a control antibodies (clone 2A3; Cat.BE0089; BioXCell) or anti-mouse CD47 antibodies (clone MIAP301; Cat.BE0270; BioXCell).

Techniques: Neutralization, Infection, Virus, Staining, Bacteria, Two Tailed Test

Journal: Nature Communications

Article Title: Airway epithelial CD47 plays a critical role in inducing influenza virus-mediated bacterial super-infection

doi: 10.1038/s41467-024-47963-5

Figure Lengend Snippet: a – j 6–8-weeks-old (18–21 g of body weight) FoxJ1 -Cre; floxed ( Cd47 Foxj1 ) and control floxed ( Cd47 f/f ) mice were infected with 100 PFU of influenza virus on day 0, and 1 ×10 8 CFU of S. aureus WT (FnBP A+/B+) and double deletion mutant (FnBP A–/B–) on day 7 ( Cd47 f/f + FnBP A+/B+, n = 9; Cd47 f/f + FnBP A–/B–, n = 11; Cd47 Foxj1 + FnBP A+/B+, n = 12; Cd47 Foxj1 +FnBP A–/B–, n = 10). Body weight loss ( a ) and survival rates ( b ) were monitored for 29 days. The dotted line indicates the body weight exclusion cut-off. A mantel cox survival analysis was used to compare the survival rates between groups. The numbers within circles or squares represent the count of surviving mice. Representative hematoxylin and eosin (H&E) staining of lung sections ( c ). Lung injury scores are presented as violin plots ( Cd47 f/f + FnBP A+/B+, n = 4; Cd47 f/f + FnBP A–/B–, n = 5; Cd47 Foxj1 + FnBP A+/B+, n = 5; Cd47 Foxj1 +FnBP A–/B–, n = 5). Tissue injury parameters were measured at 24 h after bacterial infection ( Cd47 f/f + FnBP A+/B+, n = 4; Cd47 f/f + FnBP A–/B–, n = 5; Cd47 Foxj1 + FnBP A+/B+, n = 5; Cd47 Foxj1 +FnBP A–/B–, n = 5); total cell number in BAL fluids (BALF) ( d ), bacterial adherence ( e ) and invasion ( f ) in the lung, and bacterial burden in the spleen ( g ), total protein concentrations in BALF ( h ), and inflammatory cytokines TNF-α ( i ) and IL-6 ( j ) in BALF. k Proposed model of viral infection-induced CD47-mediated secondary bacterial infection. Data are presented as mean values ± SEM. Significance was determined by one-way ANOVA with Tukey’s multiple comparisons test. Source data are provided as a Source Data file.

Article Snippet: On days 5 and 7 after viral infection, mice were injected intranasally with either IgG2a control antibodies (clone 2A3; Cat.BE0089; BioXCell) or anti-mouse CD47 antibodies (clone MIAP301; Cat.BE0270; BioXCell).

Techniques: Infection, Virus, Mutagenesis, Staining

Journal: Acta Pharmaceutica Sinica. B

Article Title: Multiparametric characterization of red blood cell physiology after hypotonic dialysis based drug encapsulation process

doi: 10.1016/j.apsb.2021.10.018

Figure Lengend Snippet: Comparison of the amount of each protein detected in RBCs, before and after encapsulation process, and with or without asparaginase (ASNase). The number of copies per cell of the proteins identified in pRBCs, proRBCs, eryaspase ( n = 4 per group) was compared between each pair of samples using a scatter plot. The Pearson correlation coefficient ( R ) was calculated for comparisons between all samples. Data for total and ghosts RBCs were separated. Some key RBCs proteins were displayed: hemoglobin proteins (HBB, HBA1), peroxiredoxin 2 (PRDX2), carbonic anhydrase (CA1, CA2), catalase (CAT), band 3 anion exchanger (SLC4A1), alpha and beta spectrin (SPTA1 and SPTB), ankyrin (ANK1), tropomyosin (TPM3), alpha and beta adducin (ADD1 and ADD2), calpain 1 catalytic subunit (CAPN1), glutathione- S -synthetase (GSS), actin (ACTB), glyceraldehyde-3-phosphate-dehydrogenase (GAPDH), Glycophorin A (GYPA), CD47.

Article Snippet: Phosphatidylserine (PS) exposure at the outer membrane leaflet of RBCs and CD47 were assessed using Annexin V-PE (Miltenyi, 130-118-363) and anti-CD47-PE antibody (Miltenyi 130-101-348), respectively.

Techniques: Comparison, Encapsulation

Journal: Acta Pharmaceutica Sinica. B

Article Title: Multiparametric characterization of red blood cell physiology after hypotonic dialysis based drug encapsulation process

doi: 10.1016/j.apsb.2021.10.018

Figure Lengend Snippet: PS exposure, CD47 expression and RBCs-EVs release before and after encapsulation process.

Article Snippet: Phosphatidylserine (PS) exposure at the outer membrane leaflet of RBCs and CD47 were assessed using Annexin V-PE (Miltenyi, 130-118-363) and anti-CD47-PE antibody (Miltenyi 130-101-348), respectively.

Techniques: Expressing, Encapsulation

Journal: Frontiers in Oncology

Article Title: Spatially resolved transcriptomics revealed local invasion-related genes in colorectal cancer

doi: 10.3389/fonc.2023.1089090

Figure Lengend Snippet: Cell differentiation trajectories in CRC obtained by pseudotime analysis. (A–D) tSNE map shows the results of the dimensionality reduction and clustering analysis of S112 (A) , S115 (B) , S114 (C) , and 927 (D) (up). Results of pseudotime cell trajectory in S112 (A) , S115 (B) , S114 (C) , and S927 (D) (down). (E) Twelve genes were screened by invasive modules. (F) The relationship of STC1 expression level and cancer stage/progression-free survival in colon cancer (up) and rectal cancer (down) from TCGA database. (G) The relationship of CES1 expression level and cancer stage in colon cancer (up) and rectal cancer (down) from TCGA database. (H) Immunohistochemical staining showed the expression of AKR1B1(left panel), STC1(middle panel), and CD47(right panel) in Mucosa and cancer(up) and Invasive margin(down) (n=45). The scale bars on the lower right are 100 µm. *P < 0.05, **P < 0.01 and ***P < 0.001. NS, not significant difference.

Article Snippet: Antibodies used include STC1 (1:50; Proteintech, China), RPL5(1:800; Proteintech, China), AKR1B1(1:50; BOSTER, China), HLA-A(1:100; BOSTER, China) and CD47(1:400; BOSTER, China).

Techniques: Cell Differentiation, Expressing, Immunohistochemical staining, Staining

Journal: Cancers

Article Title: Extracellular Vesicles Derived from Human Umbilical Cord Mesenchymal Stromal Cells as an Efficient Nanocarrier to Deliver siRNA or Drug to Pancreatic Cancer Cells

doi: 10.3390/cancers15112901

Figure Lengend Snippet: Phenotypical characterization of UC-MSCs by flow cytometry (unfilled histogram = CTRL/filled histogram = labeled cells). Histograms show positivity for the tetraspanins CD9, CD63 and CD81, as well as the MSC markers CD73, CD166, CD146, CD105, HLA-ABC, CD47 and CD200, and negativity for the hematopoietic markers HLA-DR and CD45.

Article Snippet: Cells were harvested after detachment with TrypLE Select, washed in PBS (Miltenyi Biotec, Bergisch Gladbach, Germany) and incubated for 30 min with the following monoclonal antibodies: CD105-FITC (Ancell corporation, Bayport, NY, USA), CD73-PE (Miltenyi Biotec, Bergisch Gladbach, Germany), CD146-PC5 (Beckman Coulter, Analis, Suarlée, Belgium), CD166-PE (BD Biosciences, Erembodegem, Belgium), CD45-PC7 (BD Biosciences, Erembodegem, Belgium), HLA-ABC- PC5 (BioLegend, San Diego, CA, USA), HLA-DR-PC5 (Beckman Coulter, Analis, Suarlée, Belgium), CD47-APC (Miltenyi Biotec, Bergisch Gladbach, Germany) and CD200-PC7 (BD).

Techniques: Flow Cytometry, Labeling

Journal: Cancers

Article Title: Extracellular Vesicles Derived from Human Umbilical Cord Mesenchymal Stromal Cells as an Efficient Nanocarrier to Deliver siRNA or Drug to Pancreatic Cancer Cells

doi: 10.3390/cancers15112901

Figure Lengend Snippet: Phenotypical characterization of EVs derived from hUC-MSCs by flow cytometry (black line = CTRL, red line = labeled cells). Histograms show positivity for the tetraspanins CD9, CD63 and CD81, as well as for the MSC markers CD73, CD166, CD146, CD105, HLA-ABC, CD47 and CD200, and negativity for the hematopoietic markers HLA-DR and CD45.

Article Snippet: Cells were harvested after detachment with TrypLE Select, washed in PBS (Miltenyi Biotec, Bergisch Gladbach, Germany) and incubated for 30 min with the following monoclonal antibodies: CD105-FITC (Ancell corporation, Bayport, NY, USA), CD73-PE (Miltenyi Biotec, Bergisch Gladbach, Germany), CD146-PC5 (Beckman Coulter, Analis, Suarlée, Belgium), CD166-PE (BD Biosciences, Erembodegem, Belgium), CD45-PC7 (BD Biosciences, Erembodegem, Belgium), HLA-ABC- PC5 (BioLegend, San Diego, CA, USA), HLA-DR-PC5 (Beckman Coulter, Analis, Suarlée, Belgium), CD47-APC (Miltenyi Biotec, Bergisch Gladbach, Germany) and CD200-PC7 (BD).

Techniques: Derivative Assay, Flow Cytometry, Labeling

Journal: Oncoimmunology

Article Title: IRE1α overexpression in malignant cells limits tumor progression by inducing an anti-cancer immune response

doi: 10.1080/2162402X.2022.2116844

Figure Lengend Snippet:

Article Snippet: PE-Vio770 anti-CD47 , REAfinity, Miltenyi Biotec , 130–103-105, RRID:AB_2659751.

Techniques:

Journal: STAR Protocols

Article Title: Protocol to characterize immune cell subpopulations in cerebrospinal fluid of patients with neuroinflammatory diseases using mass cytometry

doi: 10.1016/j.xpro.2024.103038

Figure Lengend Snippet:

Article Snippet: Human CD47(CC2C6)-209Bi (1:200) , Standard BioTools , Cat#3209004B.

Techniques: Recombinant, Labeling, Blocking Assay, Software, Cytometry, Flow Cytometry, Single-cell Analysis, Gene Expression